Dear Alagappan,

you try with half of full MS media and also try with different concentration of GA3 use in the MS media.

ALL THE BESt 

Thanks everybody. That was very helpful.

Dear Alagappan and Kalpesh,

Sorry, but GA3 does not work for tomato seeds, but GA4+7 does, see the link to our publication. You will need at least 1 mM GA3, while GA4+7 works in the µM range. You need to know why your seeds do not germinate. If it is dormancy you can apply different treatments, including those described in the publication. Take also care about the salt concentration in your medium.

Succes

Thank you for your response.