Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Hi, Good day! Do you have related studies about my title, "Factors Affecting the Senior High School Students' Stress Tolerance in Problem Solving"?

I am new researcher. I want to learn more and I need your help. Thank you.

04 February 2021 6,834 4 View

What are the current trends in sustainable/responsible marketing?

I am currently writing my thesis on sustainable/responsible marketing and I am looking for more insights from experts (and good articles - after 2010) about the subject. Looking forward to...

12 January 2021 7,286 6 View

Is there any research paper about integrating mobile application like google classroom in teaching?

This is to support or serve as a guide for my thesis regarding 21st century teaching strategies. Thankyou so much in advance!

07 December 2020 8,440 4 View

Efficiently transfecting HeLa Cells?

I am currently looking to transfect HeLa cells using lentivirus and Lipofectamine 2000 and have been looking for cited protocols. Would anyone have any that they are willing to share?

31 October 2020 5,639 2 View

How to compute the effects in half fractions?

Greetings, I'm doing a screening design and want to allow for lack of fit. I need replications so I want to add four additional points to my dataset. Rather than use center runs I use corner...

11 October 2020 9,672 1 View

Which of the ff. solvent is more effective to use as a bactericidal agent in antimicrobial testing of staphylococcus species, ethanol or methanol?

The antimicrobial activity testing of staphylococcus species.

02 October 2020 6,996 7 View

How to get pure recombinant virus with plaque purification ?

We are working on getting pure desired gene transfected virus. We used YFP fluorescences tags to distinguish the virus with or without gene replaced. Using PCR testing, we always have wild type...

01 August 2020 2,087 1 View

What types of research methodologies involve working with or observing people, but do not require IRB approval?

I am interested in understanding more about NGOs that work with refugees. I am brainstorming a project that does not involve IRB approval.

31 July 2020 1,854 5 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

What should a smart water system look like?

Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...

16 February 2021 1,094 3 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

Can anyone help to convert ODK XLS into XML?

We want to conduct a verbal autopsy using WHO adult VA updated tools in ODK. The current version of WHO adult verbal autopsy tools is v1.5.3. I have successfully converted VA ODK-XLS v1.4 into XML...

03 February 2021 844 2 View

I want some expert (free) advice and guidance in interpreting depositional environment from well logs in a deltaic setting. Anyone ready to help?

I am looking into the geophysical well logs from a coaliferous basin in India. I need some help in depicting the depositional environment from well logs.

02 February 2021 6,052 3 View

What new can be added in the field of historical studies to implement more realistic programs?

What new can be added in the field of historical studies to implement more realistic programs in dealing with the events of history and interpreting them with scientific sincerity and competence?

26 January 2021 559 1 View

Is Juniper Publisher ( Journal of Gynecology and Women's Health) a good journal to publish my paper?

Dear colleagues, Do you have any knowledge about Juniper Publishers? Have any of you published in the Journal of Gynecology and Women's Health ( ISSN: 2474-7602, Impact Factor: 0.621 (2017-18))? I...

21 January 2021 9,362 4 View