I'm currently writing my thesis on the MALDI-TOF-MS of apple condensed tannins, and aimed to purify plant extract via Sephadex LH-20, using a wash of 4x 90:10 methanol/water, then 4x 70:30 acetone/water. I noticed a significant removal of sugars, as expected, however signals for galloylated condensed tannins were noticeably increased relative to the ungalloylated condensed tannins. I am struggling to rationalise this observation, could anyone provide any insight into the mechanism of separation via Sephadex LH-20?
Sephadex LH-20 separates condensed tannins based on molecular size and interactions with the resin, using solvent gradients like methanol/water and acetone/water. The increased signals for galloylated tannins may result from their larger size or altered hydrophobicity, which affects their elution relative to ungalloylated forms.
Condensed tannins (also known as proanthocyanidins) separate on Sephadex LH-20 primarily based on size and hydrophobicity. It's a combination of gel filtration (size exclusion) and adsorption (hydrophobic interactions).
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