I prepared the powder of methanolic extract than I want to desolved for testing this extract on the fungus . How to desolved it whith water or with in other solvent .???
With water try a bit of DMSO or acetonitrile (missible solvent), but don't forget to have same control XP with DMSO or acetonitrile alone in water.
Hello,
In my opinion, prepare stock solution by using DMSO and further dilution can possible in water. and remember about preparing same dilluent control during analysis as mention in earlier comment.
If sample in not complete soluble in DMSO then try little sonication.
Other option you can prepare sample with different ration of water and DMSO.
Good luck!!
I aqueous extract of 100 gr of powdered plant material in one liter of distilled water, I then got the concentration of extract
Hi Nasrine,
In my opinion, DMSO is quite toxic already, so if you need to use the solution for a biological test, be sure you also check the activity of DMSO alone. In my experience, you can solubilize you MeOH extract with a solution of MeOH:DCM (1:1) and a quick sonication. If necessary, some drops of DMSO, but again, DMSO can be a problem, especially if you want to re-dry your extract after.
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