I managed to stop my PCR run after only 30 min at 16 C incubation. I found it out after 30 min and started the incubation again (16 C for 1h and 72 C 20 min) Do you think I can still use my samples? Thanks!
Yes your internal control samples should indicate if anything has gone wrong and pcr reagents are very stable ( they spend a long time at 95c) so an extra 30 mins at 16 and a short while at room temperature should not be a serious problem to the pcr working
what were the parameters of PCR? Incubation was carried out at 16C and 72C?
These are not PCR parameters! Can you give the composition of the reaction mixture?
Sorry, the components of the synthesis mix are lyophilized so they are not known to me. I decided to continue with the experiment so I will see tomorrow how the preliminar results will look like
Everything went fine with my process. It seems I managed to save my samples
- Badges
- Science topic
- Similar topics
- Biological Science
- Kinesiology
- Running