How could you use TLC for measuring drug concentration, or would this be used for seeing the stability of the drug in an extemporaneous preparation
For this purpose you can use HPTLC, which works similar to other method.
Thank you Neeraj, we only have HPLC machines so I will be using this to measure the concentration. What could be the potential uses of just TLC (not HPTLC) in respect to testing a extemporaneous drug preparation?
Simple TLC is only used for qulitative purpose and you can not aply it for any quantitative determination.
If you can find a special coloring agent to your drug, it is possible to measure concentration. For this purpose, after rising of mobile phase, add the exact quantity of agent. Then, separate the colored spots and dissolved in suitable solvent. After that you can measure absorbence and compare with standard.
I think it can be used for indirect quantitative analysis. You need to scratch off the isolate and dissolve it in a suitable solvent. This applies only for known isolates with standard UV parameters; you should consider the limit of detection. Needless to say, HPLC is direct and always the best.
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