How could one differentiate between a covalent bond, hydrogen bond and coordinate covalent bond in 13C and 1H NMR?

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What is NNFI in confirmatory factor analysis, my LT1 and CFI values are more than .9 can I conclude that model is fit?

30 June 2024 5,598 2 View

Q1: How can I improve hybridization and the process of hybridization?

Procedure: Streptavidin-magnetic bead suspension (50 µL) was transferred into a vial. The beads were washed twice by the binding/washing buffer and then re-suspended in 100µL of the buffer. A...

16 May 2024 893 0 View

How to get research papers with their cites, and what are the keywords you have to put to get context/articles/papers you need?

06 May 2024 8,904 1 View

How to create unique visualizations for a manuscript? Reference attached?

Hi A paper titled " Intelligent Computing: The Latest Advances, Challenges, and Future" was recommended by my colleague. It has unique visualizations. I am attaching one of them for reference....

29 January 2024 3,880 1 View

How to retain viral injected fluorescence in paraffin embedded brain tissue in mice and rat?

We have injected GFP and td-tomato viral tracing injection in the mice brains to see axonal projections, and we are using tertiary butyl alcohol instead of ethanol for the dehydration process as...

09 October 2023 8,654 8 View

How to covert HCN gas emission from mg/kg to mg/Nm3??

Unit conversion for HCN from mg/Kg to mg/Nm3

27 September 2023 1,414 1 View

How to increase gain property in powerlab ADinstrument?

Hi everyone, I am doing auditory brainstem response measurements using powerlab AD instruments, but I am not getting the responses for lowest dB level as they are too weak to be measured by...

13 August 2023 3,223 0 View

For confocal microscopy, is there a way to tag ubiquitin protein without hampering its job in vivo?

Hello everyone! I am planning an experiment where I would like to check ubiquitin transfer using confocal microscopy; thus, I intend to add a fluorophore tag to the ubiquitin molecule. I read a...

12 August 2023 7,532 1 View

Have you identified any safety-related behaviour changes among the workers from unorganised sectors after the pendamic?

In my observation, during the pandemic in 2020-21 workers from unorganised sectors learned things to survive and adopted creative ways to complete their tasks. However, people also started...

01 August 2023 2,272 0 View

Can we convert kallikrein inactivation units (KIU) into unit/mL?

Aprotinin is used as a plasmin inhibitor. 126KUI helps to inhibit plasmin. We have 126 ph. Euro. U/mL concentration of aprotinin with 0.5mL vial. How much will KIU be there in a 0.5mL vial?

14 May 2023 3,348 0 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View

Why do paired electrons atoms produce no magnetic field lines?

If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...

03 August 2024 3,927 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View