Genetic distance matrix was made with GenAlex but there is no option for Tree construction, so i would like to use MEGA to create Tree.
export your distance matrix (in "mega" format, according to GenAlEx export menu) and then change the extension of the exported file into ".meg". Finally, import the file into MEGA and draw your tree
what is your Data? Is it gen sequences? If your data is gen sequences, convert them to FASTA format and load them in Mega and make a phylogenetic tree.
export your distance matrix (in "mega" format, according to GenAlEx export menu) and then change the extension of the exported file into ".meg". Finally, import the file into MEGA and draw your tree
I appreciate you all for your reply. I already did it and published.Thanks.
Hi,
I also need to export my microsatellite genetic distance table from GenAlEx in order to make a NJ tree in Mega. I am running into two problems. 1) The option to call the data codominant data is greyed out and unavailable to select. It is currently called sequence data (which my data are not). 2) if I ignore problem 1 and export as .meg file anyway, then I open the file in Mega and attempt to convert to Mega format, I find that the file is identified as a .fasta file ( have to change the file type to 'fasta' to find it on my desktop), but indicates that the conversion process has occurred. If I exit the editor and go back to the analysis screen, click 'phylogeny' --> NJ, then identify the input as 'pairwise distance' with the matrix format 'upper right' (which is true looking at it in GenAlEx), I get an error message 'Oh no! Mega failed to parse the imput file. Command statements are not allowed in the distance data files (in line 4'. Line 4 currently says '#mega'. Is this something anyone can help with? Basically, I'm just having trouble getting my microsatellite distance matrix out of GenAlEx and into Mega (I've also tried PAUP) where I can make a tree. Thanks,
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