How can we purify polyphenols from an extract without using SPE ?

More Aouatif Benali's questions See All

What are the causes for the non detection of Fatty acids peaks on a Rtxi 5MS column?

Dear scientific community, We used to performe many separations and detections on our GC MS (Perkin elmer Clarus 580-SQ8S), but recently we can not separate fatty acids( few ones appears in...

06 June 2024 6,844 0 View

What is the alternative for Methoxamine in pyridine?

Dear scientific community we would like to know if there is any alternative for methoxamine in pyridine for GC MS derivatisations? regards

18 November 2023 6,362 0 View

What is the main cause of the formation of dodecanamides instead of methyl esters?

Hello all, We have perfomed a methylation either by using KOH-methanol transesterification or by using BF3 as catalyst , after injection using GC MS of the hexanoic phase we have fatty acids...

04 January 2023 3,297 1 View

How to purify amino acid hydrolysis extract for HPLC analysis?

Dear scientific community. In order to analyze the amino acid profil of proteic matrices we need a purification after the hydrolysis of our sample. We intend to use the Dowex 50WX8 hydrogen...

28 April 2021 10,014 5 View

Why System or Data engineering is become more important lately ?

from my experience in this field and knowledge data and system engineering is something fondamental and will be very important in next years as all our behavior and personal become IT system and...

31 August 2020 223 3 View

What's the indicators can be used to measure economic performance?

if we want to build a performance index depends on a set variables. what's the indicators can be used to measure economic performance and whats the desired values to these variables?

15 January 2019 3,217 75 View

Is it possible to study the causal relationship between the objectives of economic policy (magic square)

is it possible to study the causal relationship between the objectives of economic policy (magic square of kaldor), and which model we can use it, and which model we can work by it. referring to...

16 June 2017 3,139 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Bruce Neagle

It used to be accomplished with a liquid-liquid (LL) extraction. Check the internet or go back to the old books.

Article Polyphenol Liquid-Liquid Extraction Process Development using NRTL-SAC

Look what I found on RG!

Grzegorz Boczkaj

In addition - if you will develop conditions of polyphenols extract purification using LLE with deep eutectic solvents - it will for sure increase its "green" aspect as well as novelty :)

regards,

Aouatif Benali

Thank you all for your precious responses.

Best regards