How can we purify limestone which contain some impurities?

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I'm trying to respond to a request for a reprint. It gives me a message in a red box --"An error occurred." Nothing more. Why?

I have the file, and prefer to send it privately (because I'm not a lawyer, and don't really know what permissions I have to send or post it publicly). Please advise if possible.

30 May 2024 3,622 3 View

Probe-based qPCR not reaching plateau phase?

Hi, I'm in the process of developing a hydrolysis probe qPCR assay to quantify gene copy numbers of Microcystis 16S rDNA. In troubleshooting my cycling parameters, I'm getting resulting plots such...

15 January 2024 6,957 1 View

Nucleofection problem. What is the best buffer and the best program for nucleoporation?

I have been trying to transfect primary cells, essentially NK (CD56+) and monocytes (CD14+) using PiggyBac transposon system with no success. All cells die. I want to try some optimized protocols,...

31 October 2023 1,894 0 View

Any suggestions for extraction method for stem barks of Entada phaseoloides? and what rotational speed, time, and temperature for rotary evaporation?

There are limited research about Entada phaseoloides L. which is hard to know whether what extraction process of its stem bark. I saw few studies but they are reviews or an article

26 September 2023 3,071 0 View

What are some ways to repair cavities in stator coils?

During visual inspection, a cavity was detected in the stator coil of a hydroelectric stator. What are some proposed methods to repair this defect?

10 September 2023 5,709 1 View

Can i use Rehabilitation theory for my research topic entitled "Assessing the level of recreation program of detainees in jail" ?

Also, I have prepared other theories that can be likely used for the study, such as positive psychology theory to be used in the field of the criminal justice system. also I'm in need to help me...

09 September 2023 8,220 4 View

the expression of inflammatory genes and the secretion of inflammatory cytokines are not always correlated ?

Hello, I stimulate Cows PBMC with LPS and with an anti-inflammatory (6 hrs before the LPS) and another case (6 hrs after the LPS) Incubation time 24 hrs Results : RT Pcr decrease in the...

22 August 2023 1,956 0 View

Looking for Local and International Validators?

Good day, Everyone! I'm a second-year college student at De La Salle Araneta College, and we're seeking for both a local (Philippines) and an international validator for our thesis. The validator...

16 June 2023 8,197 6 View

How could it be possible to introduce NEUROPHILOSOPHY as a new academic discipline at the "Philosophisches Seminar" of the University of Hamburg?

Starting something new from bottom up is really difficult at the University of Hamburg. Only top-down approaches are successful for researchers who are already working in their field defined as...

28 February 2023 8,257 16 View

Why do GFP-positive cells die within a week after FACS?

Hi there, we are trying to establish stable transfected NIH 3T3 or C2C12 cells by FACS sorting (on plastic dishes), and starting off with 90 % green cells after the sort, we end up with hardly...

08 December 2022 1,674 4 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...

23 July 2024 3,490 6 View

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS?

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...

22 July 2024 5,334 6 View