How much plant powder do I need for different concentration of two extracts (Aqueous and Ethanolic extract)?

Supposed I need three different concentration for two plant extracts (namely: Aqueous and Ethanolic extract), starting at 50mg/ml, then 100mg/ml then 150mg/ml. In what particular weight of plant...

31 March 2019 2,775 5 View

Does anyone have any experience running ERIC-PCR?

11 May 2016 526 39 View

How do I get the electrode surface area for CR2032?

I am trying to compute the Lithium-ion diffusion coefficient (DLi) based on the equation attached[1]. So how do I get the A; electrode surface? and also how to calculate the dE/dx part of the...

13 March 2016 4,458 3 View

Any papers that have specific gases and its composition in carbonization of coconut shell?

carbonization, coconut shell, gases, carbonizer

24 February 2016 2,412 3 View

Dissertation query, Q - method inquiry. currently running a pilot study and am falling short on the analysis. All info welcome?

Hi, I'm an Undergraduate student at the University of Chichester and I'm currently writing my dissertation. I have all my statements and my question, i have knowledge of exel and spss software...

04 January 2016 3,364 4 View

What factors affect the electrode polarization for lithium-ion batteries?

Surface area, particle size? What other possible factors reduces/increases Electrode Polarization? And how does Electrode Polarization affect affect rate-capability and battery performance?

08 December 2015 2,303 6 View

Did someone try to ablate metallic cerium?

I want to ablate metallic cerium but i'm concerned about the inflammable nature of cerium. Any suggestions? Thank you!

24 August 2014 1,285 2 View

Does anyone have information (citation) or experience about cyclic olefin copolymers (COC)?

I'm interested in preparation, chemistry (stability, solubility, water content), and characteristics (optical (fluorescent), thermal, electrical....), particularly biocompatibility. Processing...

15 June 2013 6,288 1 View

How to synthesise 2,3-dibromo-6,7-dicyanonaphthalene?

Searching for a "sustainable" syntetic route (2 - 3 steps) for 2,3-dibromo-6,7-dicyanonaphtalene. Reactants should be commercially available (low priced?), best non- or less toxic and/or easy to...

13 June 2013 5,087 3 View

Can the Higgs field explain the mass of the electron?

Just attended some very interesting lectures as part of the "Higgs Fest" here at Uppsala (http://www.kalendarium.uu.se/en?eventId=4251), but while I think I understood how protons and neutrons...

26 September 2012 569 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

09 February 2021 3,779 6 View