Hi,

Try batch extraction.

Maintain temperature below 60 degree

Concentrate under vacuum

Try  90:10 to 60:40 alcohol water

What is the LOD in extract and spent and RM

these are all some check points. still have problem please IM

All the best

file:///C:/Users/M.%20PC/Downloads/P%201.pdf

article may be helpful

Watch for temperature and pH. Ensure slightly acidic medium and constant entropy during the process (near to room temperature). You may have to forgo some yield as to maintain the total extract more 0.3% in the last wash.  All the best

May be due to temperature fluctuation...

Dear Sonika

Your problem could provide peraphs of  oxidation of molecules. You can use water extraction.  Monitor  efficient temperature at 20 to 90 °C and  from 5 to 60 min for extraction times.

Hping to be hlepful

Marius