Ficoll 400 only available in market Concentrations of 50% (w/v) can be attained in water.
Ficoll should be added slowly with constant stirring.
the solubility of Ficoll 400 is at 1 g in 10 ml of
deionized water yielding a clear to slightly hazy,
colorless to faint yellow solution. Ficoll is stable in
alkaline and neutral solutions. At pH values below 3, it
is rapidly hydrolyzed, particularly at elevated
temperatures. so take 5 g in 100ml of water will give you 25% solution
How would you prepare different gradients of Ficoll of 23%, 20% and 11% from Ficoll type 400 of 5gm, Himedia?
The solubility of ficoll 400 is 1g in 10 ml, which would be a 10% weight to volume ratio.
So 5g in 100 ml would be 50% saturated.
2.5 grams in 100 ml would be 25% saturated.
1.25 grams in 100 ml would be 12.5% saturated
% saturation is how we vary the density in the gradient.
I usually prepare the 24% ficoll solution in H2O by heating (60-70°C) and mixing time to time (30-60 min?).
Dear Luke Mclaughlin
You have suggested that 1gm in 10 ml gives 10% solution is understood. But 5gm in 100ml will give 5% not 50% (w/v). So 2.5 gm in 100 ml will give 2.5 %.(w/v)
I've made 30% solutions (w/v; 30g / 100mL) as that was a good density for my 5x agarose gel loading dye. The people that are googling solubility and saying that it is 10% are incorrect. Solutions up to about 50% can be made (50 grams / 100 mL). When dealing with solutions where a significant fraction is your solute, one can't ignore the volume of your solute. Don't just add 50 grams to 100 mL of buffer; begin adding it slowly to a smaller volume and when the Ficoll is in solution, bring the volume up to the desired final volume. For preparation of density gradients, accuracy here is critical.
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