You should refer to the work of Shirreffs & Maughan RJ. (1997) Whole body sweat collection in man: an improved method with preliminary data on electrolyte content. Journal of Applied Physiology 82: 336-341
60 years ago I used palmar sweat on my Ph. D. thesis at Illinois. I think we treated paper with tannic acid and then had a gauge to measure how black the paper was. We used this measure to indicate how much the individual perspired. It is written up in (1957). Group pressure, attitude change, and autonomic involvement. Journal of Social Psychology, 45, 299-312. With Ross Stagner.
To test seat loss i would measure the body weight of the participant before the test. Then performing the cycling test ( e.g. 1 hr cycling,.....), no drinking allowed during the test. Finally, take the body weight again?
Erich, that method provides you with an indication of total water loss and assumes negiligible fluid loss from insensible sources (e.g. expired water vapour). The method of Shirreffs and Maughan also provides an opportuntity to determine whole body sweat composition.