How can I solve the high impedance problem of saturated calomel reference electrode?

The reference electrod I use is saturated calomel electrode (3M KCl as the filling solution). I got overload problem while measuring the EIS, so then I checked the impedance of the electrod and...

27 May 2020 7,557 12 View

What can cause cracks on the surface of an alloy after anodic oxidation?

We observe some cracks among the nanotubes which are formed on the surface of the Ti alloy after anodic oxdation. What could be the cause of this problem?

13 January 2020 3,086 9 View

Is it possible to transfect NBTII cells?

I am currently trying to transfect NBTII cells (wt) with lipofectamine 2000 in Opti-MEM. I tried several things already; such as splitting them 24h or 48h after transfection (I always changed the...

05 June 2018 1,845 2 View

Higher R square and less significant variables?

Im working on panel data and when I performed fixed effects model I get high r square and less significant variables. All the underlying assumptions of regression are satisfied. Kindly let me...

31 December 2017 6,577 7 View

Organizational Stroke: Why Is School Reform Not Working?

01 January 1970 7,039 13 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View