How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Hello everyone, good day! In the process of deproteinization of samples to obtain chitin, can I make use of a phenol as a deproteinizing agent?

I would like to know what other reagents can be used to obtain chitin by deproteinizing a sample. Commonly found on articles is the employment of NaOH concentrations as well as KOH. I would like...

16 October 2020 537 6 View

Can anyone please provide information in regards to the utilization of the exoskeleton as a perturbative tool for gait/balance therapy?

There exists a considerable body of literature on the application of exoskeleton as an assistive tool in the area of gait or balance therapy. I am interested in finding out more about the...

06 October 2020 9,317 3 View

What are the equation and conversion factor to estimate protein content from mushroom?

Total nitrogen was calculated with Kjeldahl method, I found different equations and factors ''Total nitrogen content of shrimp waste was determined using the Kjeldahl method. Separately, pure...

17 August 2020 4,572 9 View

How can I clean insect exoskeleton?

I want to remove whole soft tissues and maintain intact the exoeskeleton

10 March 2020 4,345 6 View

Why Calcofluor white stain fungal cells walls?

Calcofluor white (CW) is recommended to stain plant cell wall that has B1-4 Glucan linkage. On the other hand fungal chitin has Different composition. Thus, I was wondering if anyone can give me...

01 February 2020 6,120 2 View

Can anyone suggest a suitable deuterated solvent to dissolve chitin for NMR analyses?

Can anyone suggest a suitable deuterated solvent to dissolve chitin for NMR analyses?

11 December 2019 9,695 10 View

How can I easily determine the purity of my chitin ?

I extracted chitin with chemical method, now I would like to know approximatly the efficiency of my method and know for example how much proteins and minerals are left. I would like to find a...

10 November 2019 8,937 3 View

What is an appropriate solvent for chitin?

I want to inject the chitin solution into the foliage of lettuce and measure a possible difference is reactive oxygen species production. Conventional solvents are often toxic or corrosive which...

10 November 2019 743 3 View

What is an appropriate solvent for chitin?

I want to inject the chitin solution into the foliage of lettuce and measure a possible difference is reactive oxygen species production. Conventional solvents are often toxic or corrosive which...

06 November 2019 8,552 4 View