How can we isolate active compounds from aqueous extract without partitions?

More Veeramuthu Duraipandiyan's questions See All

Can anyone identify this shallow water jelly ?

The sample was collected during seagrass survey at 5 meter depth

02 August 2018 6,098 7 View

What modalities are you currently considering for this project?

Great project indeed, Dr Bigler and Dr Shenton and team.We started a similar project in early 2015, did a small pilot project (

12 February 2017 6,211 0 View

Can anybody help me to identify this benthic specimen ?

Collected from Gulf of Mannar Island,India.

22 September 2016 6,050 8 View

Can any body identify this benthic fauna?

sampling location Palkbay

20 July 2016 6,783 3 View

Is Intraoperative Neuropsychological Assessment and Brain Mapping routinely performed in your neurosurgical centres?

Greetings everyone. I am just trying to figure out how routinely intraoperative neuropsychological and brain mapping protocols are performed at highly specialised neurosurgical units/ centres ....

11 April 2016 8,986 9 View

What is the force required to cause facial and cranial bone fractures?

I am looking for the actual forces required to cause fractures to the various different regions of the facial and skull bones. If you could share some info/ literature on that would be of great...

04 August 2015 8,200 6 View

What are the biophysics or biomechanics of maxillofacial injuries in mTBI and its influence over cognitive performance & white matter tract integrity?

We recently concluded a study on the prevalence and characteristics of maxillofacial injuries in patients with mTBi, and analysed the possible influence of maxillofacial (MF) trauma over...

29 July 2015 360 5 View

Why do mild TBI patients with axonal injury do worse than patients with cortical injury in neurcognitve tasks at 6 months post trauma follow-up?

My current data on neuropsychological performance in patients with mTBI across two time points (admission vs follow up) indicate that although the patients with the cortical injury do...

27 April 2015 6,216 14 View

How soon can the demyelinating process start in mild traumatic brain injury?

Traumatic brain injury, even in its mildest form, is known to result in degenerative processes including demyelination and dysmyelination of the axons over time. The shearing and tearing of the...

20 March 2015 9,677 10 View

How does the genetic polymorphism of Brain Derived Neurotrophic Factors (BDNF) modulate cerebral oedema in traumatic brain injury?

Some of the missense mutations of BDNF have been theoretically implicated (rs6265 in particular) to influence various outcomes of brain injury. Can someone please elaborate on how certain genetic...

06 February 2015 9,232 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Can any one let me know the PDE value for Tert-Butylamine in order to control in the API?

PDE value for Tert-Butylamine

24 July 2024 1,166 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...

23 July 2024 3,490 6 View

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS?

What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...

22 July 2024 5,334 6 View

Biomerieux API Campy performance?

Hello, I have a somewhat weird question: has anyone had problems with campylobacter growing in the second half of the strip GLU through ERO? Have you managed to correct it? There is some...

19 July 2024 3,094 2 View

Interpreting ATR-FTIR Spectra for Microplastics Research?

Hello everyone, I am currently pursuing a PhD in Environmental Engineering with a focus on microplastic studies. My background is in Civil Engineering, and I am relatively new to working with...

18 July 2024 7,258 3 View

Robin Joshi

through column chromatography. use low solvent polarity to high solvent polarity. Then u can easily isolate or flash chromatography, prepHPLC etc.

Veeramuthu Duraipandiyan

Thank you for reply, there is no limitation to isolate specific compound, all compounds

I think better to go FPLC , but if there not facility then what?

Senthil Kuppusamy

If you know about your compound properties, go for crystallization technique