I have been doing some desorption experiments of PAHs from artificially contaminated soils in serum bottles supplemented with mineral media under both abiotic and biotic (bacterial inocula added) conditions. I have conducted the experiment over 32 days and at various time intervals I have collected the bottles and analyzed for residual concentration of the PAHs both in the aqueous and soil phases. From previous experiments, I am sure about the capability of the bacteria to degrade the PAHs from the aqueous phase. Is there any way to investigate whether the bacteria is capable of uptaking the PAHs directly from soil ? Do I need to do some modelling/experimentation ?
Thanks in advance
Prasenjit Ghosh
Ph.D. scholar, IIT Bombay, India
According to my knowledge the easiest way is to perform biological respiration tests with soil and PAHs use as a substrate.
Best regards
Vit
The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum impacted ecosystems. For details consult https://www.ncbi.nlm.nih.gov
Respirometry of the bacterial populations with the PAH as sole or part content of the substrate is the quickest way to assess this.
We continuously apply this technology to the challenges of the secondary treatment bacteria at my facility, with reliable data outcomes on efficiencies; toxicities and optimization options.
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