@ Qasim, Zooplankton abundance can be observed by using two methods. These are the bongo plankton net and underwater acoustics method. The attached file may be useful to you.
Com um medidor de vazão? Pode explicar o que, de fato, está em dúvida? I will try to help you.
This is a very broad question... If you want, I can send you the ICES Zooplankton Methodology Manual. Just DM me ;)
1.Quantitative analysis of zooplankton is done by Sedwick Rafter Cell but due to different shape and size other methods are also applied .
Protozoan and rotifer samples were collected using a Schindler sampler at 0.5 m below the lake surface and 0.5 m above the bottom of the lake at each sampling point, and mixed into 1-L water samples. The samples were preserved with Lugol’s iodine, and precipitated for 48 h by precipitation method. Next, the supernatant was removed by siphoning and 30 mL of concentrated solution was retained (Huang 1981). Cladopoda and copepod samples were collected 20-L using a Schindler sampler at 0.5 m below the lake surface and 0.5 m above the lake bottom at each sampling point and mixed then into 20-L water samples. The samples were then filtered with a plankton net (64-mm mesh) and preserved with formaldehyde solution (4% concentration). We also precipitated the samples for 48 h, removed the supernatant by siphoning, and then retained 30 mL of concentrate. In the laboratory, we used a fluorescence microscope (BX-51, OLYMPUS, Tokyo, Japan) to identify species and record the number of zooplankton in the samples. We used the wet-weight method was used to evaluate zooplankton biomass, which was expressed as the number of individuals per liter (ind L1 ) (Zuo et al. 2003).
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