Use Folin-ciolalteu reagent assay and calculate the phenolic content of wine as gallic acid equivalent

Thanks Mr. Ajay would u please provide me the protocol. I will be very thankful

Pravin Ojha

You can find the Folin-Ciocalteau method here:

Singleton, V. L., Citation Classic Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. Current Contents/Agriculture Biology & Environmental Sciences 1985, (48), 18-18.

Then, you should adapt the method to your samples.

please get the pdf

Article A new phenylethanoid glucoside from Jacaranda mimosifolia

I like Waterhouse micro method.  Minimises reagent required and hazardous waste produced.

http://waterhouse.ucdavis.edu/faqs/folin-ciocalteau-micro-method-for-total-phenol-in-wine

thanks everyone