After completing the complexing reaction of potassium humate with single super phosphate, what is the procedure to determine the extent of complexing happened.
Is there a method to increase the extent of humic complexing
The useage of 'complexation' in chemistry has a particular meaning that relates to electron acceptors and a donors. Your appear to be asking about humic acid and phosphate: both are potential electron donors. What is the proposed linking cation?
That makes sense. Why not use a Ca ion selective electrode to measure free Ca activity in ligand competition experiments. I don't know what spectroscopic measures may be useful. The cation could also be varied and I'd imagine pH would be an important variable. You may need to consider bicarbonate and dissolved CO2 unless they are excluded.
Another thought. Single 'super' is a mixture of Ca sulfate and phosphate. Do not assume that the sulfate is a bystander. Would you consider running a comprehensive equilibrium chemical speciation model?