Anoikis means the dying cells after the detachment from the basement membrane
I am working on transparent/translucent zirconia-based ceramics. In many papers, I have seen that they are reporting both In-line transmission (%) & Total transmission (%) value of...
20 February 2021 9,405 6 View
Hello everyone, I want to perform some gaussian calculations and want to use Materials studio for my research work. Can anyone guide me how to get access to supercomputer resources in India for...
15 February 2021 8,264 3 View
Dear community, I am currently writing my master's dissertation. As a part of the method, I have to include views (interview) of experts for the empirical analysis. The problem is I have not...
31 January 2021 3,438 3 View
Am in Peptide / Protein Research, may i know what useful analytical information we are getting from 13C & 1H NMR spectroscopy analysis? Somebody getting NMR for smaller proteins, any useful...
05 January 2021 1,196 7 View
Suggest some good articles on Genomics of this new COVID strain.
03 January 2021 7,635 13 View
I have checked annealing temperature through gradient PCR. I have checked my primer in NCBI. I have checked PCR reagents concentration. Please suggest me anyone thanks
12 December 2020 1,969 2 View
Please guide me in solving the structure and preparing a CIF file out of the results, I have only powder xrd data which I have attached here. I already have EXPO2014 and fox. Both these software...
06 December 2020 641 8 View
Hello everyone, Please help me to create a CIF with two different molecules which are linked through hydrogen bonding in the same crystal lattice. I am attaching an example to understand what I...
16 November 2020 8,056 3 View
Is it possible to perform gaussian calculations using molecular structure downloaded from chemspider or pubchem? The active pharmaceutical ingredient I am working has complex structure and I am...
29 October 2020 3,942 3 View
In high pressure liquid chromatography (HPLC), the compound is injected through a column of different sized beads.The amount of time it takes for the compound to pass through the column is the...
21 October 2020 8,801 3 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...
24 February 2021 5,183 1 View
I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...
24 February 2021 8,582 1 View
Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...
23 February 2021 6,266 3 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
I am working on modeling of Direct Contact Membrane Distillation system with COMSOL Multiphysics software. I have some problems with the mass transfer part of the simulation because I don't know...
14 February 2021 379 2 View
I'm trying to subculture NHBE cells from Lonza, however, the cells seem to not adhere to the new flasks after detachment. I've followed Lonza detachment protocol (2 mL/25 cm2 of Trypsin 0.05% and...
10 February 2021 196 1 View
This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....
08 February 2021 8,321 3 View
Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...
07 February 2021 5,591 4 View
Hello everyone, How are you guys doing? I'm currently working with antimicrobial peptide (AMP) project and one of our objectives is to predict the interaction of peptide against bacterial cell...
01 February 2021 1,351 3 View
