We are doing gel filtration in our lab and encountering issues related to column length, we are not getting expected results from our process. We previously used a 24ml column with appropriate elution buffer but could not elute the desired protein fraction. We are having issues in deciding the bed volume. How do we decide column length or bed volume?
You have not provided enough information to suggest a response. Select or develop a method of analysis for your sample. The process of method development will guide you to select the appropriate materials and method. Please contact your local instructor for assistance.
It is important to calculate the bed volume of your column using the formula pi (r square) h , value of pi is 22/7 column length h and its internal diameter ( radius r is half of diameter).
Can use simple scale to measure the length and diameter of your column.
Elute with 1.5 to 2 bed volumes of the elution buffer with elute all the proteins from gel filtration column .
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Column