How can we design primers for bacteria to study Q-PCR, cyber green?

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Marcela Krutova

Hi,

the rules are the same as for a design of every primer pair.

There are many free on-line tools. Try to find Syber green instead of Cyber green.

You can also read this useful text:

http://onlinelibrary.wiley.com/doi/10.1002/bmb.20461/full

Han Zhang

The same as other primers. As mentioned above, there are so many free on-line tool, such as:

https://www.genscript.com/tools/real-time-pcr-tagman-primer-design-tool

Hope it will help you!

Reza Vazifehmand

Thanks alot

Andreas Herchenröther

consider trying more than one primer pair and use the one with the best primer efficiency and melting curves of your PCR product.