it is deeply related to your aim. e.g. mucilage can be extracted with water.

Thank you sir for your reply:

Acutely i want to define that, Which extract is most potent/effective. So, their is any guidelines for fractionation of herbal seed.  

If you don't know which compounds confer the  biological activity for your assays, I would extract with methanol (good general purpose solvent for extraction). Alternatively, one can use hexane followed by methanol followed by water. Depending on the polarity of the active compound(s) you may find your activity in multiple solvents.

Also, do a literature search on the plant from which you obtained your seeds. This saves time in that you can see what has already been found  and how it was extracted and purified.

Check these links and publications for help:

https://www.researchgate.net/post/On_what_basis_can_I_choose_a_solvent_for_soxhlet_extraction_technology#55409e65f15bc7284e8b45fe

https://www.researchgate.net/post/Can_you_use_methanol_for_plant_extract_research

https://www.researchgate.net/post/Wwhich_is_better_gradually_organic_solvents_used_between_polar_to_nonpolar_used_or_non_polar_to_polar_used_for_plant_extraction

https://www.researchgate.net/post/Which_is_the_best_solvent_for_the_extraction_of_the_constituents_polar_and_non_polar_organic_compounds_of_dried_herbal_plant

http://www.slideshare.net/rahulbs89/extraction-of-plant-contituents

Thanks  B.R. Sir for your kind help. Now, i will go through these literature and hope its useful for me.