I want to add defibrinated blood to my blood agar base medium.
Hi Abhijit,
It is an easy method and you can use it routinely in your lab - you need to aliquot a define amount of the blood in centrifuge tube (50 ml or in your choice), add some glass beads and start shaking intensively for about 5 min by hand.
Or the expensive version is -
http://www.freepatentsonline.com/4129131.html
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