I want to examine effect of calcium deficiency in fertility of female mouse. i need a protocol for creating calcium deficiency.
It requires to discuss with me
Hi Fatemeh
See this link
http://www.hindawi.com/journals/bmri/2015/604275/
And this
http://jultika.oulu.fi/files/isbn9514270355.pdf
Or you can give anti estrogen to the mice like tamoxifen, Ethamoxytriphetol, clomifene citrate and Fulvestrant.
Regards
Ali
You have to treat animals with calcium deficient food and water available in the market. Discuss with dealers.
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