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Hey, does anyone know any effective way to separate virus from exosomes fraction? my problem with the separation of the EVs in this system is the fact that the virus that we work with particles...

06 December 2020 4,765 2 View

Number of cells for extacellular vesicles extraction?

hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you

03 December 2020 6,488 2 View

What are the XRD analysis conditions of the cement, sand and Limestone ?

I am a Phd student and I need to do XRD analysis on my pouder samples namely ( cement- sand- limestone and calcined clay). I was asked asked to specify the analysis conditions: -the nature of...

07 October 2020 5,737 6 View

Can anyone recommend me some articles about Fire endurance of wood concrete?

Are there documentations about Fire endurance of wood or wood shavings concrete? The literature is not very clear about this .

12 August 2020 8,203 3 View

Is it possible to use plant extract as natural sterilizer?

For economic reasons, sterilzation against covid_19 is very necessary, so is it possible to use plant extract?

11 May 2020 2,674 14 View

Which statistical analysis is more appropriate for my study?

I am trying to find the relationship between the use of defence mechanisms in early adulthood as predictors of neuroticism. Those who use more defence mechanisms will score higher on the...

14 April 2020 3,353 7 View

How do I carry out performance evaluation on a sorting machine?

I design a prototype for sorting tomatoes based on size and colours. The prototype will sort big red, big green, small green and small red

12 March 2020 2,386 3 View

Preparation of the mix for Standard PCR (without using a kit)?

Hi, Please anyone already prepared the mix for Standard PCR? But without using a commercial kit. It is for staphylococcal enterotoxins genes. I am looking for the concentrations of each...

08 February 2020 4,487 6 View

How to deal with mental health issues?

I feel communication helps in coping such issues to some level.

16 August 2019 7,405 31 View

Course -Preparation advices !?

Dear colleagues, As I am new in the domain of teaching courses, so it takes too much of time to prepare my course, saying for example: cryptography course, web development,..etc,... So, my...

30 May 2019 6,236 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View