Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

What is the difference between actin and loading control in normalization of western blot???

Regarding normalization of western blot analysis, I have found different strategies people follow but I couldn't understand the principle and which one is the accurate, 1- some refer to loading...

04 December 2020 5,721 8 View

Detecting bands by ImageJ on mac not fixed properly in each lane???

I am using ImageJ on mac and when I select bands, the first one detected correctly, when I click cmd 2 (= ctr 2 on windows system) to detect the second band, the rectangle gets fixed on the...

01 December 2020 9,538 4 View

My question is about how to determined MIC concentration in the use of microdilution method?

I would like to ask you about MIC first, after I searching in the internet about my query I could not found any answer. My question is about how can I calculate the concentration of stock...

23 November 2020 6,737 3 View

Can you please help me with organic acid HPLC analysis?

Hello everyone, I am experiencing difficulties in the HPLC analysis of adipic acid. I have tried Atlantis C18, mobile phase phosphoric acid solution PH 2.65, flow rate 0.7 ml/min, column temp 35...

20 November 2020 8,280 4 View

Is THC causing underlying paranoia in individuals?

In other words, is there a chemical impact caused by THC which could be activating neural correlates in the insular cortex (ic) of the brain? The increase in the sensory activating...

04 August 2020 967 4 View

Is vitamin C beneficial against Corona virus?

Vitamin C (ascorbic acid) is a water-soluble vitamin that is thought to have beneficial effects in patients with severe and critical illnesses. It is an antioxidant and free radical scavenger that...

04 August 2020 3,856 98 View

How long does the coronavirus stay on your clothes?

03 August 2020 1,674 96 View

Are health workers at risk from a novel coronavirus?

Are health workers at risk from a novel coronavirus? Yes, they can be, as health care workers come into contact with patients more often than the general public WHO recommends that health care...

27 July 2020 1,027 97 View

How should I analyze the multiple scans in Malvern Zetasizer software?

Hello, I am currently using the Malvern Zetasizer software for the purpose of obtaining the size distribution of nanoparticles produced in the lab. When I perform the runs in the software to get...

15 February 2021 4,251 1 View

Which optimization algorithm is most efficient for fitting a regression model using training dataset?

The goal of regression model is either to minimize the prediction error or to maximize a classification accuracy depending on the type of the model. Is there any optimization algorithm that is...

10 February 2021 4,128 3 View

Why does the accuracy of NN decreases when two datasets are combined?

I am working on gait analysis using a feed-forward NN. When I use data from the accelerometer (3 columns for each axis if stored in a data frame), I get an accuracy of 95%, and when I used data...

07 February 2021 9,654 6 View

Which techniques are used to improve the accuracy of deep learning models by compressing neural networks?

Hi all, I am looking for enhancing the accuracy of a deep learning model using some techniques to compress neural networks. Could someone suggest to me some of the tools/techniques,...

29 January 2021 1,781 4 View

When is the right time to stop training a classification algorithm?

In this example giving 400 samples looks like there is an accuracy of 91.5% while training beyond that point, accuracy is getting decreased. Are 400 samples enough to keep for this example? If...

27 January 2021 5,657 3 View

45% top1 Accuracy on TinyImgeNet?

I trained a model from scratch no transfer learning of any kind I got top1 validation accuracy of 45.79 and top5 validation accuracy of 72.39. I did a lot of hyperparameters tuning I have seen...

19 January 2021 6,883 2 View

How to express accuracy of the neural network where the dataset has not been split into two, training set and test set?

In use, the weights obtained are considered with the greatest accuracy when the data set is divided, because the weights are initialized at random and the training data is swapped at random...

19 January 2021 5,714 4 View

Multicollinearity between bioclimatic variables in spatial distribution modelling?

Is there any manual guide to perform multicollinearity test between environmental (19 Bioclimatic) variables for MaxEnt modelling? Using all variables without removing the correlated one affects...

14 January 2021 3,807 7 View

Why do I have good performances for the test set but bad performances in predicting new data ?

Hello :) I'am trying to predict churn for the year of 2020 based on historical data from 2014 to 2019 on R Studio. The aim is to predict churn probability for every person. At the beginning...

13 January 2021 5,565 7 View

How to change an internal parameter during MATLAB fminseach?

An optimization is performed using the MATLAB fminseach function. This optimization has a parameter that adjusts the precision, and the larger the value, the wider the search range of solution...

12 January 2021 3,832 1 View