how the effect of mass transfer in HPLC can be lowred by at lower linear velocities?
The effects of mass transfer are lower at lower linear velocity of the mobile phase.Please refer the following aritcle
Ref: www.chromacademy.com/lms/.../Theory_Of_HPLC_Band_Broadening....
In HPLC, the analyte is sorbed to the solid particle's surface and consequently the analytes closed to the surface are depleted (i.e. concentration is decreased). To supply the constant concentration, a flow is required. Higher the fluid flow faster the analyte supply rate (i.e. mass transfer rate). Now, the depletion effect can be minimized if the particle diameter is very low and/or the surface area of the particle is high. In this case, the surface will get enough analyte from surrounding volume without the need of high flow rate. Attached paper explained the fundamentals.
http://www.nature.com/nbt/journal/v26/n4/abs/nbt1388.html
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