Salam Mahdi , i guess your question can be solve by my colleague Dr . Seenaa , contact with her, she will help you . Seenaa Badr Mohammed

Regard ..

Hello Al-Hareth Mahdi ,

I understand your query this way, You have calculated the fold gene expression of your samples at each time points using 2 del del CT method.

If this is correct ,

I would suggest you to plot a line graph of each sample with fold expression on Y-axis and Time points as X-axis.

I guess you are trying to find a optimum time point and concentration for your desired action for which

This would give you a clean representation of dependence/varation in fold gene expression at each time point between different samples(treatments)