I want to assess the impact of burning biomass on soil carbon storage and CO2 emission into the atmosphere. Does anyone have any suggestions? Perhaps any protocols or literature?
What do you mean by burning biomass? Aboveground biomass? The simplest way would be to measure the total soil C and organic C before and after burning to determine organic carbon loss (or comparing burned and unburned plots). For measuring CO2 emission into the atmosphere, I don't have any specific protocols but this has been a major topic in Global Change research and you could look at the IPCC reports and global change literature for suggestions.
All references (Spain worked at lot on this topic: CARBALLAS, ALMENDROS; VALLEJO, etc.) said that the usual forest fires DO NOT influence too much on the quality & quantity of the SOM, because they affect only the first -2 cm soil depth.
Only extreme, static fires can affect the soil if remain too much time in the site burning big amounts of biomass, but this is not usual situation.
Forest fires burn usually very quickly dry stand litter and leaves or needles in the canopy, reamining trunks and soil slighty affected. Only some special, intense fires can burn trunks and in this situation we can loss a significant part of SOM, in special if they is scarcely humified; this is the case of mars and peats.
Then, kind of fires detemines the influence of forest fires on soil & then SOM.
Dennis, you need to know the difference in the amount of carbon in the soil ie C sequestered . before and after the burning. Directly to your query. To convert C to CO2. From IPCC (2007)
CO2 = amount of Carbon sequestered X 3.75 divide by 1000.
3.75 equals molecular weight of CO2 divided by atomic weight of carbon. IPCC does not use 44 and 12, respectively, but the weighted average of molecules containing the several carbon isotopes found in the atmosphere, mainly 12C and 13C.
I do not know where the 1000 presented by Oku comes from: you are converting grams (or Mg...) of C to grams etc of CO2, not to express anything per thousand.
CO2/C ratio is 3,67, then, the amount of isotopes considered seems to be significant. Obviously, the factor 1000 could be a question of units. It seems to refer to a conversion, probably from g C m-2 to Mg C ha-1 (in this case a '0' is lacking) or similar transformation.
Which approach to chose is quite dependant on what type of ecosystem you're interested in. Measuring soil respiration rates before and after a fire would be one way to go but that is of course only possible in the case where you're planning a burning and not really for natural fires.Eddy covariance (EC) measurements would integrate CO2 fluxes over larger areas and does not require a lot of manual work. If you're ecosystem is a dense forest, understory EC measurements would be difficult while it would be an excellent choice for shublands, grasslands etc. Chamber measurements of soil respiration is probably a better choice in most cases but it is labor intensive and you'll face problems of scaling up your results to the ecosystem level.
One thing to keep in mind is that if all or a major part of the aboveground vegetation disappears in the fire, the belowground autotrophic respiration (root excudates etc) will stop/decrease and cause a change in total soil respiration rates, which might make it difficult to interpret what happened to the heterothrophic soil respiration rates.
Combining soil respiration measurements with measurements of soil organic carbon before and after a fire is recommended but not straightforward, I think. SOC might increase after the fire as a consequence of a lot of dead organic material being deposited on the soil surface during the fire. You'll also need a lof of samples for scaling up and that is labor intensive and expensive.
I've attached two references for you. I also think there has been some work on carbon fluxes in relation to fires at the Hyytiälä site in Finland. You should be able to find some references from those experiments.
Ma et al (2004) - Short-Term Effects of Experimental Burning and Thinning on Soil Respiration in an Old-Growth, Mixed-Conifer Forest
Ryu et al (2009) - Prescribed burning and mechanical thinning effects on belowground conditions and soil respiration in a mixed-conifer forest, California