The condition of a specific land site that is known to have high lead content near Laguna de Bay, Philippines will be simulated considering conditions such as sunlight, soil pH, oxygen/carbon dioxide ratio, and other abiotic factors. There will be an experimental group for which Pleurotus ostreatus, known for its properties in mycoremediation,will be cultivated for one month in the artificial environment of soil with high lead content, and another group that acts as the control.
I would like to know how to efficiently use the two techniques, atomic absorption spectroscopy (AAS), and x-ray fluorescence (XRF) in determining and visualizing the lead content of the simulated soil before and after cultivating P. ostreatus for a month, as well as the oyster mushroom itself.
dear Angelo,
you may have success if you are able to work minimizing the errors due to contaminations. You would like to use 2 methods, why? Both are working quantitatively when calibrating in an adequate form... what do you mean with visualization? nether nor is giving a picture ... ??
For the plants, I suppose that you will have not only one Pb concentration for nutrition, and that you will make at least 5 different concentrations and 5 replicates of each. Do not underestimate statistics.
Alex
keep in mind that X-RF is weak in terms of detection limit and always captures the total Pb content of a soil. Keep also in mind that you stickto wavelength dispersive X-Rf and to consider interferring elements like As. Getting reasonable accurate and precise Pb concentrations with X-RF requires a lot of experience with the method. Bowse or literature on RG, there are some papers about it. With AAs you get what you have extracted, like Syamkmar already said, but even if you go for a "total" digest you never get values as high as with a precise X-RF, no combustion method is "total". If you want to assess biological available Pb you have to use weaker extractants and they may drop you out of the detection limits of normal flame AAS, says require graphite furnace techniques or better ICP.
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