How can one make a haploid yeast diploid without mating?

01 January 2016 1 4K Report

Are there any chemical methods/agents available to make haploid yeast diploid without mating it with opposite mating type yeast ?

Please advise.

Thank you

Herve Levesque

may be by performing a spheroblast fusion? a colchicine treatment? followed by cell dimension selection?

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