I plan to determine if a certain liquid fungicide applied to a bored hole in a tree trunk will produce adverse effects on the tree. The said hole reaches until the sapwood, and is created by drilling. Some studies I have come across measured growth inhibition in seedlings and rates of photosynthesis in leaves. What could be possible indicators in the case of tree trunks?
I have a strong feeling that lichens do not like fungicides. So quantify the number of lichens on a unit surface trunk in function of the application of your fungicide, and the answer will soon be yes or no for lichens as indicators for fungicide application. You will have to do this experiment as well in function of air pollution levels, because lichens are very sensitive to air pollution as well.
Cheers,
Frank
Please set up your experiment to investigate at least five rates of concentration of your liquid fungicide and record the parameters you mentioned and those mentioned by the other contributors and analyse you results and see nature of the response.
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