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How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

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How to write a paper method for interview in a qualitative research ?

Hello, Currently, I am writing my master thesis proposal. I have been to provide a method paper for (question creation and interview evaluation). It's my first time planning to conduct...

27 April 2024 4,710 1 View

A question about antibodies?

I am hoping that someone can help me find an antibody for GABA, GAD 65 or GAD 67 that works well in marmoset cerebral cortex Thanks

21 February 2024 7,493 2 View

Where to Find Dataset of Requirements Gathering Scripts and User Stories Extracted from Requirements?

Hello, I am looking for a dataset of requirements gathering scripts and user stories extracted from those requirements. Anything similar would be helpful. Thank you.

01 February 2024 4,604 0 View

Please fill my research questionnaire ?

Hello , I hope this message finds you well. I'm a final-year civil engineering student working on a dissertation about the sustainability of ancient Egyptian construction techniques and their...

14 November 2023 987 1 View

How can I confirm the western blot band is identical with my target protein?

I used some polyclonal western blot antibody, and that datasheet size is 45-50kDa I also get the band in expected size range, but there's multiple other band(at least 3 bands in other size), and...

25 July 2023 5,349 5 View

[PROCESS Macro for SPSS] What are the Reference and Counterfactual Values of X?

Hi! I am trying to run a model 4 mediation with the xmint option (what was once model 74) but PROCESS produces the following error: "A reference value of X is required for this model." I've...

24 May 2023 6,965 2 View

Can I get some cell seeding/plating help?

Hello, I would some help with seeding/plating cells. I counted cells using the hemocytometer. I got 1,025,000 cells per mL. I need to seed 250 cells per well for 36 wells and each well has...

26 March 2023 2,481 3 View

Tritox-100 permeabilzation for immunocytochemistry?

what is the recomended concentration and duration to permeabilize mitochondrial membrane for ICC?

26 March 2023 1,958 9 View

Will a signal peptide still be cleaved if GFP is fused in front of it?

I am studying the subcellular localization of a protein. To see if a GFP fusion protein can be compared to the native protein, I fused GFP to the N-terminal of the protein. In my western blots and...

01 March 2023 749 0 View

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