Dear Weam,

You can use the DPPH scavenging activity method.

The procedure of this mehod is as follows:

* first of all, you make the standard curve of Ascorbic acid by using 5-7 different Ascorbic acid solutions. The scavenging activity of both Ascorbic acid solutions and your food sample (extract) could be measured as follows:

* 500 ul of sample extract (or Ascorbic acid solution) is mixed with 2.5 ml of 6×10^-5 M methanolic solution of DPPH.

* The reaction mixture is

incubated in the dark at room temperature for 90 min.

* then the absorbance is measured by spectrophotometry at 515 nm.

*The DPPH scavenging activity value of sample (or Ascorbic acid solution) (%) = 1- ( Absorbance of sample/absorbance of control)×100

Control sample contains all reagents excluding the extract.

* By the previous equation you first draw the curve of DPPH scavenging activity of Ascorbic acid. Then, from this curve and by using the absorbance value of your sample you can find the DPPH scavenging activity of your sample.

Best wishes

Duried

Dear Weam,

You can use the DPPH scavenging activity method.

The procedure of this mehod is as follows:

* first of all, you make the standard curve of Ascorbic acid by using 5-7 different Ascorbic acid solutions. The scavenging activity of both Ascorbic acid solutions and your food sample (extract) could be measured as follows:

* 500 ul of sample extract (or Ascorbic acid solution) is mixed with 2.5 ml of 6×10^-5 M methanolic solution of DPPH.

* The reaction mixture is

incubated in the dark at room temperature for 90 min.

* then the absorbance is measured by spectrophotometry at 515 nm.

*The DPPH scavenging activity value of sample (or Ascorbic acid solution) (%) = 1- ( Absorbance of sample/absorbance of control)×100

Control sample contains all reagents excluding the extract.

* By the previous equation you first draw the curve of DPPH scavenging activity of Ascorbic acid. Then, from this curve and by using the absorbance value of your sample you can find the DPPH scavenging activity of your sample.

Best wishes

Duried

DPPH radical scavenging or Reducing power assay coul be used.

Thank you @Jorge carlos Ruiz Ruiz