Question for SAS with two Factor?

I have experimented with two Factor: Factor A: Two iron sources and Factor B: two seasons with three blocks. When I run the SAS, the interaction not significant, but the combination of the...

18 May 2020 2,576 6 View

Can anyone send a Guidelines to write a systematic review in agriculture topic?

I need Guidelines to write a systematic review in agriculture topic.

24 March 2020 8,050 8 View

How to convert mg/L to mg/kg values in the measurement of plant tissue minerals analysis?

I analyzed the plant tissue minerals such as N,P,K by atomic absorption (AAS). I take 0.25g of dry sample and digested. Then filtered and complete the solution to 100ml. The final reading for N,...

23 February 2020 3,164 8 View

What the differences between small scale aquaponics and low tech aquaponics?

What the differences between small scale aquaponics production and low tech aquaponic production in concept, mechanism, components and production.

09 October 2019 1,082 2 View

What the ideal methods for analysis Chlorophyll a and b, and Anthocyanin for hydroponics lettuce???

I need the full protocol for analysis Chlorophyll a and b, and Anthocyanin for hydroponics lettuce and the ideal solvent for analysis such as acetone for cholophyll and methanol for anthocyanin.

06 July 2019 6,017 3 View

I used Hanna instruments (Multi parameters) and I used for meaurement Nitrate. The readying was more that 30 mg/l. How I can diluted the water sample?

I want to diluted water sample. The sample volume(10 ml), the reading give me out of range( the range 30 mg/l). amount of Nitrate I have more than 30 mg/L. How i can dilute the water sample to get...

04 May 2019 9,246 12 View

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04 May 2019 3,065 1 View

I used Hanna instruments (Multi parameters) and I used for meaurement Nitrate. The readying was more that 30 mg/l. How I can diluted the water sample?

I want to diluted water sample. The sample volume(10 ml), the reading give me out of range( the range 30 mg/l). amount of Nitrate I have more than 30 mg/L. How i can dilute the water sample to get...

04 May 2019 4,390 13 View

How do I calculate the amount of acetic acid needed to lower pH from 7 to 6 of a volume in hydroponics system?

I need to reduce the pH from 7 to 6 by using acetic acid in hydroponics system?

03 April 2019 8,401 7 View

What the protocols for calculator evaporation rate from open tank in aquaculture /aquaponics???

I looking for simple methods or equation for calculation evaporation rate from open tank in aquaculture /aquaponics???

02 March 2019 1,134 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View