I want to use HS-SPME_HPLC-UV/vis to test some methods for my analytical research in our lab. My question is, could I insert the SPME fiber directly into the HPLC injector port?
Why you are not eluting the absorbed PAH from SPME fiber (probably PDMS) using ACN and transferring the eluent to LC for analysis?
I should also pinpoint that FLD is more sensitive in PAH analysis if you have and can combine it with the LC system.
Noel W Davies and İsmail Emir Akyildiz gave excellent answers. But just to add, SPME is injected directly into GC cos the injection port is heated and this causes the analytes to elute into the port as they are volatile. For HPLC you have got to inject liquid samples..but with special injection port as suggested above it is possible.
Noel W Davies, @ismail Emir Akyildiz, Benedict Odjobo Thank you all
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