Has anyone handled Liberase TL or Collagenase? I dissolve and aliquote Liberase with water. Then I free free and store the enzyme in liquid nitrogen. The question is how to thaw them to keep them active? One paper said they thaw it on ice, while other said it should be under hot water to get quick thawing. Which one is better? Liberase is similar as collagenase. Any experience using collagenase should also be useful.
We have used collagenase for years, but we always kept it freeze-dried as originally provided by the supplier, and stored at -20 ºC.
We only aliquoted and dissolved it just before using it, and only the amount that was needed for the assay.
I am sorry. I didn't explain myself clearly. We didn't get to freeze the dissolved collagenase, we dissolved it just before using it.
We prepare the LIberase (Roche including thermolysin) according to the manufacturers instruction and keep @ -20 after aliquoting everything. We thaw it just before using. Thanks
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