Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Treating Intermediate Variable in Linear Regression Analysis in SPSS?

While performing the Linear regression analysis in SPSS version 26.0.0. my intermediate/mediation variable goes to the excluded section. Please share your valuable suggestion "How to restrain the...

25 February 2021 4,591 5 View

Why are there two reduction peaks in my cyclic voltammogram?

When I measured the cyclic voltammogram of palladium in the pbs solution, two reduction peaks appeared. This did not match the literature I saw. Why? And how can I find the possible reaction...

20 February 2021 2,486 3 View

How is Initial GDP measured ?

Dear All, I am working on estimating Debt-Growth Threshold but confused about some points. My questions are; - How do we calculate initial GDP Per Capita? Shall we do it in STATA or excel file?...

15 February 2021 2,677 2 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

How can I get rid of the bubbles in the SBS membrane?

I used methylcyclohexane/ ethylcyclohexane / n-butyl acetate mixed solvent to dissolve thermoplastic SBS & terpene resin. The mixture is then poured onto a glass substrate. After solvent...

24 January 2021 8,306 3 View

Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

After G actin polymerization and labelling I didn't got filaments. Why?

Purified G actin and got 42kda band and an additional band just below it. While performing G actin polymerization and labeling with rhodamine-phalloidin I didn't observe filaments under the...

20 January 2021 3,751 2 View

How to Import .inp file from abaqus CAE to Ansys perfectly?

I am trying to import an Abaqus .inp file to Ansys but it does not import the whole assembly but only imports a single part. How to solve this.

17 January 2021 5,450 3 View

Which one I should choose in Abaqus ?

Hello, plese give me your idea! I simulate one slab concrete affected by temperature at top and bottom surfaces. after that, have a static load distribution with small area put on top surface. I...

12 January 2021 5,769 4 View

The reason of PMMA channels cracking?

07 February 2021 3,663 2 View

Lift off problem with Indium/Gold deposition?

Hi, I'm doing 5/40nm In/Au e-beam evaporation at rates 0.2A/s(for In),1A/s(Au) on lithographically(doubly coated 495k,950k PMMA e-beam resist) done Si substrate at base pressure 2e-6torr. I'm...

18 January 2021 7,550 4 View

How to choose a PMMA with the correct thickness IN E BEAM LITHO?

I try to pattern the gold layer around 2 nm-20nm on silicon, I used around 2000nm thickness (PMMA 950K A13), I tried to pattern five samples in total, but only one showed the pattern I wanted. I...

13 January 2021 7,710 1 View

Why isoelectric point (IEP) of alumina (Al2O3) dispersion decreases with the increasing of calcination temperatures?

We have prepared different alumina powders (fine/submicron-sized) from waste sources at different calcination temperatures (C.T.). From zeta potential-pH graph, we saw that IEP values decreased...

09 January 2021 9,404 3 View

Is surface embrittlement unavoidable after spray coating with brittle powder ?

I am not an expert at materials science. However, when I look at the literature, I read a process called cold spraying. This process is basically spraying a ductile powder (e.g. copper) onto a...

07 December 2020 5,378 2 View

Why is my TiO2 sol milky?

@Krunal Dholiya has sent me this question which I post below so he can get the benefit of many experts. Dear, I am preparing a thin film using TiO2 sol but i m fasing problem, with time it is...

23 November 2020 1,188 2 View

How to make few mm (~4-5 mm) thick Poly(methyl methacrylate) film?

Hi, I'm aware that thin film of PMMA can be spin coated. But I'm more interested in making few mm thick PMMA which will act as a substrate. Please share your expertise on how this can be...

21 November 2020 4,561 10 View

Where are some Chemical Vapor Deposition (CVD) and Physical Vapor Deposition (PVD) coating facilities available in India?

For my B.Tech final year project I want to test multilayer coated carbide insert and test it for tool life and wear properties. I am looking for CVD and PVD facilities that can coat tungsten...

08 November 2020 8,091 4 View

What could I expect to happen if I dilute a solution of PMMA in chlorobenzene with acetone?

I received a bottle of PMMA in solution with no information about which solvent was used, so I started using acetone to dilute the original solution. I had not experienced any consequences or...

03 November 2020 4,065 4 View

Carbon pick up between ladle and tundish?

There are lots of cases that carbon pick up is seen between ladle and tundish in our plant during continuous casting of slab steel (even more than 0.02 wt%). What is the reason of this...

01 November 2020 1,275 14 View