The following might help.

To synthesize a cisplatin-adenine biomolecular complex, you will be combining cisplatin (cis-diamminedichloroplatinum(II)) with adenine (a purine nucleobase). The procedure involves the coordination of cisplatin with the nitrogen atoms of the adenine molecule. Here’s a general methodology for this:

Materials Needed

Cisplatin (cis-diamminedichloroplatinum(II))

Adenine (purine nucleobase)

Solvents:

Water (for aqueous solutions)

Ethanol (optional, for recrystallization or washing)

Glassware: Beakers, flasks, stirring rods, etc.

pH meter or pH strips (optional, to control the pH)

Heating source (optional, if necessary for solubilization)

Step-by-Step Methodology

1. Preparation of Cisplatin Solution

Dissolve cisplatin in water to create a solution. A typical concentration would be around 10 mM. Ensure that the solution is freshly prepared and used within a short period, as cisplatin can hydrolyze over time.

Note: Cisplatin is generally dissolved in water and not in strong acidic or basic conditions to prevent decomposition of the platinum compound.

2. Preparation of Adenine Solution

Dissolve adenine in water. Adenine has relatively poor solubility in water, so you might need to heat the solution slightly (not exceeding 50°C) to achieve dissolution.

A typical concentration could be in the range of 5–10 mM.

3. Mixing Cisplatin and Adenine Solutions

Slowly add the adenine solution into the cisplatin solution with gentle stirring.

The complex will form through coordination between the nitrogen atoms of adenine and the platinum center of cisplatin.

The reaction could be slightly exothermic, so it's good to monitor the temperature.

Adjust pH (if necessary): The complex formation might be pH-dependent. If needed, adjust the pH of the solution to around 5.5–6.5 using a dilute acid or base. However, avoid extreme pH conditions, as they may cause dissociation or decomposition of cisplatin.

4. Stirring and Reaction Time

Stir the mixture for 2–4 hours at room temperature.

This allows sufficient time for the cisplatin-adenine complex to form.

Depending on the conditions, the complex may form immediately or take a little time to fully coordinate.

5. Isolation of the Complex

After the reaction is complete, you can isolate the complex by evaporating the solvent or using solvent extraction.

Precipitation: If the complex precipitates out of solution, filter it and wash it with cold ethanol or distilled water to remove unreacted adenine and other by-products.

Drying: Dry the complex under reduced pressure or in a desiccator.

6. Characterization of the Complex UV-Vis spectroscopy

To confirm the coordination of adenine to cisplatin, you can use UV-Vis spectroscopy. The absorption spectrum may show shifts corresponding to the interaction between cisplatin and adenine.

NMR spectroscopy: If you're synthesizing the complex in a suitable solvent (like D2O or other deuterated solvents), proton or 13C NMR can provide evidence of the formation of the cisplatin-adenine complex.

X-ray diffraction (XRD): If suitable crystals are formed, you may use single-crystal X-ray diffraction for detailed structural confirmation.

Mass spectrometry (MS): This can help determine the molecular weight and the stoichiometry of the complex.

Reaction Mechanism:

Cisplatin binds to adenine via the nitrogen atom(s) of the purine ring. The two chloride ions in cisplatin are displaced upon interaction with the nitrogen of adenine, leading to the formation of a coordination complex between platinum and adenine.

Possible Modifications:

Solvent Choice: In some cases, organic solvents like ethanol or methanol may help in solubilizing either the cisplatin or adenine. Be mindful of the solvent’s effect on the complex’s stability.

Temperature: The reaction can be carried out at room temperature, but mild heating may help to facilitate the complex formation.

Stoichiometry: Varying the stoichiometry of cisplatin and adenine can yield complexes with different stoichiometric ratios (e.g., 1:1, 2:1).

This methodology provides a basic route to synthesize a cisplatin-adenine biomolecular complex, and further modifications can be explored based on specific experimental conditions.

Haileyesus Gedefaw thank you so much!

Can you send me some of the latest research publications so that I can refer to them?