Hi, 

Is it soluble in water?If it is then use water as a solvent,else ethyl C2H5OH

Dear Susan

Thanks for answer. It is not soluble in water. 

[1] My friend you need a constant pH  of 7.4. For this you need a buffer called

PBS[Phosphate buffered saline] 7.4 ( Isotonic with human body)

This is available as powder( mainly) and also in solution form

[2]SSD is practically insoluble in water, in ethanol and in diethyl ether. It is freely soluble in 25 % w/v ammonia solution .

[ 3] Depeding upon the amount of SSD, add the PBS7.4 buffer

[4] If PBS 7.4 is not available, you may prepare it as follows.

There are many different ways to prepare PBS.  One of the most common preparations is described below.

For 1 liter of 1X PBS, prepare as follows:

1.                          Start with 800 ml of distilled water:

2.                          Add 8 g of NaCl.

3.                          Add 0.2 g of KCl.

4.                          Add 1.44 g of Na2HPO4.

5.                          Add 0.24 g of KH2PO4.

6.                          Adjust the pH to 7.4 with HCl.

7.                          Add distilled water to a total volume of 1 liter.

Dear Manohar

Many thanks for your extensive answer. I will try this.