In wells there is very distinct and visible the liquid-air interface
standing cultures line.
How big volume of 33% Acetic acid should be? Do i need to cover the liquid-air interface line and solve it? Or add small volume of 33% Acetic acid to avoid this line?
You can refer to the following links for getting certain valuable informations:-
1. http://www.scielo.br/pdf/bjm/v42n2/11.pdf
2. www.nature.com › Journal home › Archive › Protocol
I think that the best that you can do, is to follow the original protocol for biofilm in multiwell plates for Pseudomonas species: http://www.ncbi.nlm.nih.gov/pubmed/9632250
I hope this helps.
You can go through the following articles
1) Journal of Microbiological Methods
Volume 105, October 2014, Pages 134–140
2) Nature Protocols 5, 1460–1469 , doi:10.1038/nprot.2010.110
3) J Vis Exp. 2011; (47): 2437, doi: 10.3791/2437
4) Jundishapur J Microbiol. 2014 February; 7(2): e14358., DOI: 10.5812/jjm.14358
Take a look at the attached paper and see if the discussion makes sense for your results.
Article Biofilm Localization in the Vertical Wall of Shaking 96-Well Plates
ALI favors biofilm formation. Since you washed the plates before adding the dye, so you should add solvent (33% acetic acid) sufficient enough to dissolve all the remaining dye.
Dear Slava,
Add 125 ul 30% of glacial Acetic acid.
Thanks,
Musafer
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts