Greeting.
I have a Capillary Electrophoresis Agilent G1600 (3D CE system) that has been having problems lately. The system can not achieve stable pressure and crashes afterwards with the errors 5207 and 5210.
In the logbook, it says to check bottle and screws for fitting. I have done this and the errors still occurs.
Can some help me?
I don´t have the Service Diagnostic Disk. Can someone provide it for me? Or give me the link to it?
Thank you very much
This is a common set of error messages (5207 and 5210). In 99% of cases it means you have a pressure problem. The cause is often a bad seal (The o'ring) on the Buffer bottle cap or loose tube fitting. Check these connections and carefully clean ALL salt residues off their surfaces. Also, be sure and use the "Change Bottle" feature in the software when you release pressure in the buffer bottle so the system can reset properly. Failure to do so will also cause this error to be set. *The pumps usually last for ten years, but these older CE units are long past that date so I would always consider that the pump may be bad too (remove and check level).
Another cause could be a contaminated air inlet filter. These filters are designed to be replaced on a regular basis and I see a lot of clients ignore them (and then they are clogged with dirt). If this filter is clogged, the pump can not operate correctly.
Sorry, do not have a diagnostic disk file available to measure pressures or manipulate the valves with.
please check injector system of your system. change the septom and be sure that the liner inside the injector dos not have any crack or contamination. if it's possible change the liner too.
Besides the problems mentioned above, you might also have a faulty actuator in the pressure line. We recently had a similar problem and an Agilent technician replaced the faulty actuator that regulates the pressure. You can try to get a replacement or have a third-party to come in for repair.
I would like to thank everyone for your input.
I will check all the advices given and will update the situation by Monday or Tuesday.
Greetings everyone.
This is an update on the pressure problem.
I checked every single connection for cracks or loose fitting and all seem fine to me. The buffer bottles also seem to be fine. Nevertheless, I added Teflon to every screw for better fit. (At some stages I forgot to click on the "change bottle", maybe that's the problem?)
The air inlet filter seems ok, but I replaced it anyway.
How can I check the actuator? How do I test if it is ok?
Also, I have removed the air pump fuse, but I can not see if it is ok, because all of it is dark. Is this normal? The number on the top of it is "BUSS MDA 6 1/4 A". In attachment, I'm sending a photo of it.
Additionally, I have a leak problem now, coming from the DAD. But there seems to no humidity on the sensor, so I'm a bit startled.
Thank you very much for your contributions.
And please, help me :)
The fuse can easily be checked as good using a multimeter. Place on Ohms scale and check for continuity at both ends. Should read close to zero Ohms if good and Mega Ohms if Open or blown.
You Wrote: "At some stages I forgot to click on the "change bottle", maybe that's the problem?". It may very well be as that will cause the errors. Just click on the replenishment bottle in the GUI. This is covered in the users manual.
Leak could have been caused by movement of the parts when you checked for secure connections. The capillary is easy to break and this will cause a leak. I would carefully go over all the connections again, being sure to clean the seals and dry off the sensor before restarting.
The actuator is usually checked using the service functions. These are pretty reliable, but if the system is clogged with buffer salts over prolonged time, many valving problems can appear which take a great deal of time to troubleshoot and repair (call for service).
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