I want to determine the fatty acid profile of an oil after being hydrolyzed by an enzyme. I'm using a GC. So, to do this I have to methylate the oil (convert to FAME). But before the methylation I have to separate the FFA and the oil which supposed to contain mono, di and triglycerides. I want to discard the FFA. So how could I separate them? (using solvent extraction)
Hi,
for the seperation of FA, MG, DG, and TG I would suggest thin layer chromatography.
Good luck
Hello!
I would recommend to use one of the protocols for neutral lipid fractionation with silica columns listed here:
http://www.cyberlipid.org/cyberlip/home0001.htm
In our institute we are using the third and forth procedure and this works quite fine. Of course you should analyze the fractions afterwards with thin layer chromatography.
Greets and good luck!
I agree with Barbara: silica and amino propyl SPE cartridges work quite good:
https://www.researchgate.net/publication/220036753_Improvement_of_a_solid_phase_extraction_method_for_analysis_of_lipid_fractions_in_muscle_foods._Anal_Chim_Acta_520(1-2)201-205
Cheers
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