By changing hydrophillic-Lipophilic ration you would be able to separate two phases

I suggest to add brine for a better phase separation.

It is necessary to separate organic phase (vegetable oil + TWEEN) using simple liquid-liquid extraction. Extraction by equal volume of chloroform or hexane-chloroform mixture would be quite efficient. It should be noted that usual and well known Folch or Radin-Hara alcohol-based extraction methods must be used necessarily to extract MUCH MORE polar lipid components such as phospholipids, monoglycerides or nonesterified fatty acids (but vegetable oil almost completely consists of non-polar triglyceride components and is effectively extracted from water matrix with organic liquid of rather low polarity).

If the water and organic phases of extraction emulsion are poorly separated due to the presence of TWEEN detergent impurity, add a few drops of ethyl alcohol and, possibly, centrifuge or filter the mixture. Then organic extract is evaporated using vacuum or a weak stream of nitrogen.

The sample obtained is ALMOST pure plant triglyceride, since the detergent (TWEEN) is usually added in very small concentrations (up to 1–2% by weight of the substance to be emulsified) to produce an emulsion. Thus, the presence of detergent will have almost no effect on the results of subsequent titrimetric or chromatographic determination of plant triglycerides (the values of the own relative errors of these methods exceed the error introduced by such an insignificant impurity content).

Nevertheless, it is possible to easily separate low polar oil triglycerides from rather polar polyhydroxylated TWEEN by solid phase extraction: the sample is deposited in the cartridge prepacked with silica gel (for example, BONDELUT or other – take sorbate no more than 2% by weight of silica gel). Triglyceride is eluted quantitatively with pure chloroform thus being separated from TWEEN (polar TWEEN may be eluted only by means of much more polar chloroform-methanol mixtures).

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