Andrea Magnavacca

4 Questions 3 Answers 0 Followers

Questions related from Andrea Magnavacca

Can FBS interfere with cell treatment?

I usually perform short-term treatments (6h) and so I use serum free medium, but in a new protocol I have to treat for 5 days Caco-2 cells grown on Transwell membranes. In this case should I use...

10 October 2019 7,632 4 View

How can I separate and extract oil from pharmaceutical emulsion?

Hi everybody, I need to quantitatively separate vegetable oil from a w/o emulsion in order to determinate the oil content and perform further analysis (such as saponification) on the separated...

01 January 2019 488 3 View

How to perform qPCR relative quantification?

HI everybody, I'm performing qPCR data analysis and a colleague suggested me a sort of modification over 2^-ddCt calculation. I have the average dCt of four experiment, control and treatment. He...

09 September 2018 7,786 3 View

How can I perform statistical analysis of RT-qPCR gene expression data?

I used a commercial qPCR array to evaluate differences in the expression of various (48) genes between cells cultured in normoxia conditions vs. hypoxia conditions. I performed four replicates of...

08 August 2018 7,546 8 View