Dear Researchers,
Kindly give me information regarding,how to select the different solvent for the separation of the compounds by TLC..
Regards
Mohan Kumar S
http://orgchem.colorado.edu/Technique/Procedures/TLC/TLC.html
one of many links;
it depends on the type of material or compounds you separated. but in general polar compounds need polar solvent (water, alcohol, chloroform) and non polar compounds need non polar solvents (hexane, petroleum ether, benzene, toluene). I think that there is no fixed solvent for each test. you must optimize your system to obtain the best results
Hi, please use the data base of CBS Camag to search the possible TLC system for your compounds
http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm
It is quite simple. Just go through the polarity index of the solvent. In TLC operated as normal phase chromatography, as the polarity increases the retention factor will increase. Hence by controlling polarity, we can get the desired retention. If the Rf value is near to zero, Increase the polarity. If Rf value is near to 1, decrease the polarity.
Thank You for your valuable answers...........
But I have a doubt.........
If the compound is unknown how we can select Polar or Nonpolar solvents
Regards
Mohan Kumar s
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