Kindly help with the analytical step-by-step procedure of the quantification or a laboratory characterization technique that can be use for this purpose ?
Quantitative analysis of lignocellulose components of non -treated and steam exploded barley, oat and wheat straw using Fourier Transform Infrared Spectroscopy has proved to be a cost effective quantification of lignocellulosic components ( cellulose, hemicellulose and lignin ) in agricultural biomass was 7.5%, 2.5% and 3.8%, respectively. For more details consult https://www.pdfs.semanticscholar.org
Hi,
May check the attached files for the TAPPI standards methods to analysis lignin and holocellulose ('cellulose and hemicellulose).
Sylvester Peter Antai Thank you so much Prof, I am grateful for the response. However, the url you attached is not accessible.
NIR Spectroscopy is a very useful tool, as Mr. Peter Ansai explains, that needs to be well calibrated. In wood materials is very important to choose a very uniform way to prepare samples: sawdust or directly wood. For quantifying cellulose, all methods are long or not very accurate.
This is actually a rather difficult problem, and most methods are somewhat empirical. The problem is, you do not know exactly what the answer is to standardize your methodology. The next problem is the hemicellulose varies in nature from sample to sample. One approach is to do an acid hydrolysis. The best way to do this is with super concentrated cold HCl. The problem with hot acid is the sugars start breaking down to furfural from pentoses, and hydroxymethyl furfural from hexoses, and the latter is further reactive and makes some brown precipitate that will be confused with lignin. From the hyrolysate, you neutralize (vacuum distilling off the acid helps) and do a sugar analysis. There will be some glucose in some hemicelluloses, but for many there is not very much. The residue is lignin, and a small amount of lignin may have dissolved in the acid solution, but after neutralization the lignin can be assessed by UV spectrosopy.
It is not an easy thing to do with real accuracy. You will see a lot of reports where it is done, but you have no idea what the real answer was supposed to be.
The below mentioned researcher has many articles involving quantifying the percentage composition of Cellulose, Holocellulose, and Lignin in various Bio-material , specially the older articles. Happy new year.
https://www.researchgate.net/profile/Yehia_Fahmy
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