How can I pulverize plant leaves for use in DNA extraction?

24 October 2022 1 4K Report

Without using liquid nitrogen or electronic devices.

Gently crumble leaf tissue over cold pestle of liquid nitrogen. Grind frozen leaf with one spatula of fine sand add 0.5 spatula of PVPP powder after grinding. 3. Scrape powder into dry tube and add pre-heated buffer and mix gently.

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